Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis
Identifieur interne : 004672 ( Main/Exploration ); précédent : 004671; suivant : 004673Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis
Auteurs : David A. Garber [États-Unis] ; Stephen M. Beverley [États-Unis] ; Donald M. Coen [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.
Url:
DOI: 10.1006/viro.1993.1612
Affiliations:
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Garber</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston</wicri:cityArea></affiliation></author><author><name sortKey="Beverley, Stephen M" sort="Beverley, Stephen M" uniqKey="Beverley S" first="Stephen M." last="Beverley">Stephen M. Beverley</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston</wicri:cityArea></affiliation></author><author><name sortKey="Coen, Donald M" sort="Coen, Donald M" uniqKey="Coen D" first="Donald M." last="Coen">Donald M. Coen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">197</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="459">459</biblScope><biblScope unit="page" to="462">462</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Garber, David A" sort="Garber, David A" uniqKey="Garber D" first="David A." last="Garber">David A. Garber</name></region><name sortKey="Beverley, Stephen M" sort="Beverley, Stephen M" uniqKey="Beverley S" first="Stephen M." last="Beverley">Stephen M. Beverley</name><name sortKey="Coen, Donald M" sort="Coen, Donald M" uniqKey="Coen D" first="Donald M." last="Coen">Donald M. 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